Friedrich-Alexander-Universität Erlangen-Nürnberg

Cancer detection by a hyperspectral reflectance and multispectral fluorescence video endoscope

Cancer detection by a hyperspectral reflectance and multispectral fluorescence video endoscope

Introduction

Cancer is the second upper most killer in the world after cardiovascular related disease. Until now the respective sensitivity and positive predictive value of standard upper endoscopy for diagnosing Barrett’s Esophagus have been reported to be only 82% and 34%. In our approach we are going to combine reflectance and fluorescence for cancer detection. The overall scope of this project is to develop a prototype of video endoscopic system that is capable of acquiring multispectral fluorescence and hyperspectral reflectance images over a wide range of wavelengths in stomach through a standard flexible video endoscope for revealing pre-cancer and cancer and providing a surgical guidance.

Figure 1: Endoscopy setup

Actual State

In the actual state we finished the setup for hyperspectral reflectance and the preliminary results on phantoms we developed to simulate optical properties as it is in tissues in vivo. With this system it is possible to select a spectrum with various centre wavelengths between 400 and 630 nm with bandwidths between 20 and 30 nm. For the hyperspectral reflectance measurements eight different wavelengths were used to acquire the reflectance images and the spectra. The following biological tissue-like phantoms with following optical properties were used: μa = 3.7cm-1 and μs’ = 8.7 cm-1 for cancerous tissue and μa = 0.3 cm-1 and μs’ = 6.8 cm-1 for healthy tissue at a wavelength of 500nm.

Figure 2: Sensitivity and specificity for increasing number of adjust wavelengths and their standard deviation

Results

The first goal for these preliminary results was to get an estimation of the possible accuracy and see how many different wavelengths bands we need for optimal differentiation. With a LDA-analysis 90% sensitivity and specificity are reached(figure 1).

Mission

SAOT provides an interdisciplinary research and education program of excellence within a broad international network of distinguished experts to promote innovation and leadership in the areas

Optical Metrology
Optical Material Processing
Optics in Medicine
Optics in Communication and Information Technology
Optical Materials and Systems
and Computational Optics.