FRET spectroscopy studies of molecular dynamics of bacterial mechanosensitive channels

Mechanosensitive (MS) ion channels are to date among the best characterized biological force-sensing systems. They present an excellent example of coupling protein conformations to the mechanics of the surrounding cell membrane. Studies of MS channels conducted close to 30 years now have greatly contributed to our understanding of molecular mechanisms underlying the physiology and biophysics of mechanosensory transduction. On the example of bacterial MS channels this lecture will demonstrate how fluorescence spectroscopy (FRET, FLIM, patch fluorimetry) has been used for studies of lipid-protein interactions in MS channels.