Approach for Qualitative Tissue Differentiation Using Laser Induced Breakdown Spectroscopy (LIBS)

Abstract: The main goal of this study was to identify different tissue types during laser ablation which will provide a real time feedback mechanism for clinical Laser surgery applications to significantly improve the application of the technique. Laser Induced Breakdown Spectroscopy (LIBS) by monitoring the plasma plume created during laser surgery processes is used to qualitatively differentiate tissues. Experiments on tissue samples of bone, fat, muscle, nerve and skin were performed using a high power laser capable of generating plasma from the samples. Atomic emissions from the plasma generated were collected using a spectrometer. The collected spectra were analyzed using statistical analysis methods, Principal Component Analysis (PCA), Linear Discriminant Analysis (LDA) and Receiver Operating Characteristic (ROC), for the classification of the spectra to their respective tissues. Successful tissue differentiation results were obtained promising the application of the technique in providing real time qualitative tissue information for a laser surgery feedback system.